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宿主来源
Rat抗原名称
CD3分子别名
T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD247; Cd3z; Tcrz细胞定位
Cell membraneAccession
P24161克隆号
17A2抗体类型
Rat mAb抗体同种型
IgG2b应用
FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Ms |
The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. CD3 is associated with an heterodimer creating the T-cell receptor (TCR). The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines.
流式分析
Flow cytometric analysis of Mouse CD3 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD19 antibody and SDT Biotin Rat Anti-Mouse CD3 Antibody at 5 μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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