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宿主来源
Rabbit抗原名称
PCNA分子别名
Proliferating cell nuclear antigen; Cyclin免疫原
Synthetic Peptide细胞定位
NucleusAccession
P12004克隆号
SDT-297-116抗体类型
Recombinant mAb抗体同种型
IgG应用
ICFCM, ICC反应种属 ?
Hu, Ms, Rt阳性样本
HeLa预测反应种属
(反应种属缩写表)Av, Bv, Hm纯化方式
Protein A浓度
2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:200 | Hu |
| ICFCM | 1:800 | Hu |
Proliferating cell nuclear antigen (PCNA) is a type of DNA clamp that acts as a DNA polymerase in eukaryotic cells δ. The processing factor of is crucial for replication. PCNA is a homologous trimer that achieves its processability by surrounding DNA, where it serves as a scaffold for recruiting proteins involved in DNA replication, DNA repair, chromatin remodeling, and epigenetics. It is expressed in most tumors as a proliferative antigen.
流式分析
Flow cytometric analysis of PCNA expression on 4% PFA fixed 90% methanol permeabilized HeLa. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) was stained with FITC Rabbit IgG Isotype Control (Black line histogram) and SDT PCNA Recombinant Rabbit mAb (FITC Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- PCNA (FITC Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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