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宿主来源
Mouse抗原名称
α-tubulin分子别名
Tubulin alpha-4A chain; Alpha-tubulin 1免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
P68366克隆号
S-364-23HL抗体类型
Recombinant mAb抗体同种型
IgG1,k应用
ICFCM ,ICC ?反应种属 ?
Hu, Ms, Rt阳性样本
HeLa预测反应种属
(反应种属缩写表)Bv, SeUr, Pg, Lob, Cz, Pl, Fs, Dr, Ar, Xe, Hm, Av纯化方式
Protein G浓度
2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:100 | Hu |
| ICFCM | 1:800 | Hu |
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.
流式分析
Flow cytometric analysis of α-tubulin expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) was stained with FITC Rabbit IgG Isotype Control (Black line histogram) and SDT α-tubulin Recombinant Rabbit mAb (FITC Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-α-tubulin (FITC Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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