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Rabbit抗原名称
Vinculin分子别名
Metavinculin (MV); VCL免疫原
Synthetic Peptide细胞定位
Cytoskeleton, Cell membrane, Cell junctionAccession
P18206克隆号
S-1635-6抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, NIH/3T3, C6预测反应种属
(反应种属缩写表)Bv, Av, Xe纯化方式
Protein A浓度
2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
ICC ?
稀释度
应用 稀释度 推荐种属 ICC 1:50 Hu, Ms, Rt
Vinculin is a critical cytoskeletal protein that plays a significant role in cell adhesion, migration, and mechanical force transmission. It is highly enriched in regions where cells contact one another and the underlying substratum. Vinculin is involved in the formation and stabilization of focal adhesions, which are essential for cell-matrix interactions. It binds to several proteins, including talin, α-actinin, and paxillin, to link the actin cytoskeleton to integrins, facilitating cell adhesion and migration. Vinculin helps in transmitting mechanical forces from the extracellular matrix to the actin cytoskeleton, playing a crucial role in mechanotransduction. It can undergo conformational changes upon binding to different partners, which is essential for its function in force transmission. Vinculin stimulates actin polymerization and recruits actin remodeling proteins, which is vital for cell protrusion and migration.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Vinculin (FITC Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Anti- Vinculin (FITC Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti- Vinculin (FITC Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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