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宿主来源
Rabbit免疫原
Recombinant Protein抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A标记
Agarose beads性状
Liquid缓冲体系
50% slurry in PBS pH7.4, 0.05% Proclin 300. Ensure thorough mixing before each use. Wide-mouth pipette tips are recommended. In case of difficulty in aspiration due to improper operation, additional Storage Buffer can be added at your discretion.储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
Protein purification
IP
Rabbit Anti-V5 Agarose Beads is an affinity chromatography medium designed for easy, one-step purification of recombinant V5 tag fusion proteins in E. coli, insect cells and mammalian cells. The recombinant V5 tag fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-V5 Agarose Beads. It is the excellent choice for high performance purifications.
电泳
Rabbit Anti-V5 Agarose Beads is used to immunoprecipitate V5-tagged fusion proteins from Escherichia coli lysate supernatant. During elution with Peptide elution buffer, the enriched V5-tagged fusion proteins are released from Rabbit Anti-V5 Agarose Beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: Flow-through
Lane 4: V5-tagged fusion protein
Lane 5: V5-tagged fusion protein







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