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宿主来源
Rabbit免疫原
Recombinant Protein抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A标记
Agarose beads性状
Liquid缓冲体系
50% slurry in PBS pH7.4, 0.05% Proclin 300. Ensure thorough mixing before each use. Wide-mouth pipette tips are recommended. In case of difficulty in aspiration due to improper operation, additional Storage Buffer can be added at your discretion.储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
Protein purification
ChIP
IP
Rabbit Anti-HA Agarose Beads is an affinity chromatography medium designed for easy, one-step purification of recombinant HA tag fusion proteins in E. coli, insect cells and mammalian cells. The recombinant HA tag fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-HA Agarose Beads. It is the excellent choice for high performance purifications.
电泳
Rabbit Anti-HA Agarose Beads is used to immunoprecipitate HA-tagged fusion proteins from Escherichia coli lysate supernatant. During elution with Peptide elution buffer, the enriched HA-tagged fusion proteins are released from Rabbit Anti-HA Agarose Beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: Flow-through
Lane 4: HA-tagged fusion protein
Lane 5: HA-tagged fusion protein
ChIP
Chromatin immunoprecipitation (ChIP) was performed on 293F cells were either untransfected (left panel) or transfected with an HA-tagged human H3 construct (right panel) cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Rabbit Anti-HA tag agarose beads, H3 beads and Rabbit lgG lsotype Control agarose beads for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA) showed the enrichment of RPL30, MYOD1 and SAT-α in Rabbit Rabbit Anti-HA tag agarose beads-immunoprecipitated sample.
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