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宿主来源
Mouse抗原名称
CD123分子别名
Translation initiation factor eIF2 assembly protein; Cell division cycle protein 123 homolog (Protein D123); HT-1080; PZ32细胞定位
Cytoplasm, Cell membraneAccession
O75794克隆号
7G3抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
RhMk, CyMk, Hu阳性样本
human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu, RhMk, CyMk
The CD123 protein, also known as the Interleukin-3 receptor subunit alpha (IL-3RA) or CD123, is a crucial component of the interleukin-3 (IL-3) receptor complex and belongs to the Type I cytokine receptor family, specifically the Class 5 subfamily. CD123 serves as the high-affinity receptor for IL-3, forming a heterodimeric receptor complex with the β chain (CSF2RB). This protein mediates the stimulatory effects of IL-3 on the proliferation and differentiation of various hematopoietic cells, including pluripotent hematopoietic stem cells and lineage-committed cells. CD123 is expressed on normal plasmacytoid dendritic cells, basophils, monocytes, and eosinophils, and is overexpressed in various hematologic malignancies. In clinical research, the expression of CD123 is associated with chemotherapy resistance and high-risk genetic alterations in certain leukemias, such as acute myeloid leukemia (AML).
流式分析
Flow cytometric analysis of human CD123 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 605™ Mouse Anti-Human HLA/DR antibody and either APC Mouse IgG2a, κ Isotype Control (Left panel) or SDT APC Mouse Anti-Human CD123 Antibody (Right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of CD123 expression on Rhesus monkey peripheral blood cells. Rhesus macaque peripheral blood cells were stained with APC Mouse IgG2a, κ Isotype Control (left panel) or SDT APC Mouse Anti- Human CD123 Antibody (right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD123 expression on Cynomolgus monkey peripheral blood cells. Cynomolgus macaque peripheral blood cells were stained with APC Mouse IgG2a, κ Isotype Control (left panel) or SDT APC Mouse Anti- Human CD123 Antibody (right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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