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产品中心 抗体 琼脂糖/磁珠偶联抗体

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Rabbit Anti-HA Tag Magnetic Agarose

价格 ￥2,824.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1719
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    HA tag

  • 免疫原

    Synthetic Peptide

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Species Independent

  • 纯化方式

    Protein A

  • 标记

    Magnetic Agarose

  • 性状

    Liquid

  • 缓冲体系

    20% slurry in PBS pH7.4, 0.05% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 应用

    Protein purification

    Co-IP

    ChIP

    IP

背景介绍

  • Rabbit Anti-HA Tag Magnetic Agarose is an affinity chromatography medium designed for easy, one-step purification of recombinant HA tag fusion proteins in E. coli, insect cells and mammalian cells. The recombinant HA tag fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-HA Tag Magnetic Agarose. It is the excellent choice for high performance purifications.

  • 电泳

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on 293F cells were either untransfected (left panel) or transfected with an HA-tagged human H3 construct (right panel) cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Rabbit Anti-HA Tag Magnetic Agarose, H3 beads and Rabbit IgG Isotype Control Magnetic Agarose for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA)
      showed the enrichment of RPL30, MYOD1 and SAT-α in
      Rabbit Anti-HA Tag Magnetic Agarose-immunoprecipitated
      sample.

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