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宿主来源
Mouse抗原名称
CD14分子别名
Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein免疫原
Recombinant Protein细胞定位
Secreted, Cell membraneAccession
P08571克隆号
S-631-50抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 0.1μg per million cells in 100μl volume | Hu |
CD14 (cluster of differentiation 14) is a human protein made mostly by macrophages as part of the innate immune system. It helps to detect bacteria in the body by binding lipopolysaccharide (LPS), a pathogen-associated molecular pattern (PAMP). CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol (GPI) tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa).
流式分析
Flow cytometric analysis of Human CD14 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with SDT Biotin Mouse Anti- Human CD14 Antibody at 0.1 μg/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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