分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
p27分子别名
Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1; Cdkn1b; KIP-1细胞定位
Cytoplasm, NucleusAccession
P46414克隆号
SDT-R350抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
IF ?
稀释度
应用 稀释度 推荐种属 IF 1:50 Hu, Ms, Rt
p27 Kip1 is a member of Cip/Kip family of proteins, leading to cell cycle arrest in G1 phase. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung. In pathology, it can be used for the identification of thyroid adenoma and thyroid follicular carcinoma.
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human testis. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse testis. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat testis. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
评论(0)