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Rabbit抗原名称
CD23分子别名
BLAST-2, C-type lectin domain family 4 member J,Fc-epsilon-RII,Immunoglobulin E-binding factor, Lymphocyte IgE receptor, FCER2; CD23A,CLEC4J,FCE2, IGEBF免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P06734克隆号
SDT-028-67抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
IF ?
稀释度
应用 稀释度 推荐种属 IF 1:50 Hu
CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. CD23 is known to have a role of transportation in antibody feedback regulation. Antigens which enter the blood stream can be captured by antigen specific IgE antibodies. The IgE immune complexes that are formed bind to CD23 molecules on B cells, and are transported to the B cell follicles of the spleen. In flow cytometry, CD23 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell lymphoma (CD23-negative). CD23 can also be demonstrated in germinal centre follicular dendritic cells using immunohistochemistry but is minimally expressed by benign germinal center B cells.
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD23 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human kidney. Anti-CD23 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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