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宿主来源
Rat抗原名称
CD45分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); T200, PTPRC; Lymphocyte antigen 5 (Ly-5)细胞定位
Cell membraneAccession
P06800克隆号
S-R477抗体类型
Rat mAb抗体同种型
IgG2b,k应用
FCM反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
0.1mg/ml标记
APC-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 2.5μl per million cells in 100μl volume | Ms |
CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
流式分析
Flow cytometric analysis of Mouse CD45 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with either APC-Cy7 Rat IgG2b Isotype Control (Black line histogram) or SDT APC-Cy7 Rat Anti-Mouse CD45 antibody (Red line histogram) at 0.25 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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