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Rabbit抗原名称
GAPDH分子别名
Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH免疫原
Synthetic Peptide细胞定位
Membrane, Cytoplasm, NucleusAccession
P04406, P16858克隆号
S-240-147抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Zf, Hu, Ms, Rt阳性样本
HeLa, MCF7, NIH/3T3, mouse brain,C6, rat brain, zebrafish预测反应种属
(反应种属缩写表)Hm, Mi, Pg, Sq, Dg, Ct, Sh, Qu, Bv, Ck纯化方式
Protein A浓度
1 mg/ml标记
HRP性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:5000-1:10000 | Hu, Ms, Rt |
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH comprises a polypeptide chain of 335 amino acids. Structural studies identified two regions, namely the glyceraldehyde-3-phosphate catalytic site and the NAD+ binding site, a primary structure known as the Rossmann fold, which is also required for the activity of other dehydrogenases. Many of these roles are dependent on the ability of GAPDH to bind different macromolecules in the cell.
免疫印迹
WB result of GAPDH Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: GAPDH Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Predicted MW: 37 kDa
Observed MW: 37 kDaWB result of GAPDH Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: GAPDH Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Predicted MW: 37 kDa
Observed MW: 37 kDaWB result of GAPDH Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: GAPDH Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Predicted MW: 37 kDa
Observed MW: 37 kDa
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