大包装询价 产品介绍 引用文献(0) 评论(0)
宿主来源
Mouse抗原名称
CD326分子别名
Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein (EGP); Epithelial glycoprotein 314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1细胞定位
Cell membraneAccession
P16422克隆号
S-R464抗体类型
Mouse mAb抗体同种型
IgG2b应用
FCM反应种属 ?
Hu阳性样本
HT-29纯化方式
Protein A浓度
50μg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5 μl per million cells in 100μl volume | Hu |
CD326, also known as EpCAM (Epithelial Cell Adhesion Molecule), is a transmembrane protein expressed in epithelial cells. It plays a role not only in normal epithelial cells, contributing to embryonic development and adult cell differentiation, but also in epithelial-derived cancer cells, significantly impacting tumor formation and spread. CD326 also plays a role in the tumor microenvironment, participating in interactions between tumor cells and stromal components, cell migration, cell differentiation, and signal transduction. In clinical applications, CD326 is considered a potential diagnostic and therapeutic target due to its expression in epithelial-derived malignant tumors. For example, CD326 can be used for the positive selection of viable epithelial tumor cells in the peripheral blood, bone marrow, lymphatic tissues, and serous effusions of cancer patients. Additionally, the expression levels of CD326 in certain tumor types are associated with advanced disease stages and poorer overall survival rates, indicating that it may be a potential prognostic biomarker.
流式分析
Flow cytometric analysis of Human CD326 expression on HT-29 cells. Cells from the HT-29 (Human colorectal adenocarcinoma epithelial cell, Right) or Jurkat (Human T cell leukemia T lymphocyte, Left) was stained with FITC Mouse IgG2b, κ Isotype Control (Black line histogram) and SDT FITC Mouse Anti-Human CD326 antibody (Red line histogram) at 0.25 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
实验方案
引用文献(0)
评论(0)