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Rabbit抗原名称
NaK-ATPase分子别名
Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1免疫原
Synthetic Peptide细胞定位
Cell membrane, MelanosomeAccession
P05023克隆号
S-1373-39抗体类型
Recombinant mAb应用
IF反应种属 ?
Hu纯化方式
Protein A标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| IF | 1:200 | Hu |
NaK-ATPase, also known as the sodium-potassium adenosine triphosphatase (Na+/K+-ATPase) or simply the sodium pump, is a crucial transmembrane protein found in the plasma membrane of animal cells. Utilizing the energy released from ATP hydrolysis, Na+/K+-ATPase actively transports Na+ ions out of the cell against their concentration gradient while simultaneously transporting K+ ions into the cell. This ion transport activity is essential for maintaining the electrochemical gradients across the cell membrane, contributing to the stability of the intracellular and extracellular environments. In addition to its role as an ion pump, Na+/K+-ATPase has signaling functions. It can serve as a receptor for certain hormones and drugs, participating in intracellular signaling cascades.
免疫荧光
IF shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- NaK-ATPase antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- NaK-ATPase antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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