产品介绍 评论(0)
宿主来源
Rabbit免疫原
Synthetic Peptide抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A标记
Magnetic Agarose性状
Liquid缓冲体系
20% slurry in PBS pH7.4, 0.05% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
Co-IP ,Protein purification ,IP稀释度
Rabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose is an anti-FLAG Rabbit Recombinant monoclonal antibody covalently conjugated to magnetic agarose beads. It can be widely used for immunoprecipitation or purification of fusion proteins or their protein complexes with Flag tags.
电泳
Rabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose is used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with Acidic elution buffer, the enriched FLAG-tagged fusion proteins are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: FLAG-tagged fusion protein
Lane 4: Rabbit IgG Isotype Control Magnetic AgaroseRabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose is used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with Peptide elution buffer, the enriched FLAG-tagged fusion proteins are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: FLAG-tagged fusion proteinRabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose is used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with 2x SDS-sample buffer, the enriched FLAG-tagged fusion proteins and antibodies are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: FLAG-tagged fusion protein

评论(0)