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宿主来源
Rabbit免疫原
Recombinant Protein抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Species Independent纯化方式
Protein A标记
Agarose beads性状
Liquid缓冲体系
50% slurry in PBS pH7.4, 0.05% Proclin 300. Ensure thorough mixing before each use. Wide-mouth pipette tips are recommended. In case of difficulty in aspiration due to improper operation, additional Storage Buffer can be added at your discretion.储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
Co-IP
Protein purification
IP
Rabbit Anti-GST agarose Beads is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-GST agarose Beads. It is the excellent choice for high performance purifications.
电泳
Rabbit Anti -GST agarose Beads were used to immunoprecipitate GST (glutathione S-transferase) from E.coli system.
During elution with Acidic elution buffer buffer, the enriched GST fusion protein are released from the agarose beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: RFP fusion protein.
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