Alexa Fluor® 488 Mouse Anti-Human CD16 Antibody (S-R440)
Low affinity immunoglobulin gamma Fc region receptor III-A,IgG Fc receptor III-A,CD16-II,CD16a antigen,Fc-gamma RIII-alpha (Fc-gamma RIII,Fc-gamma RIIIa,FcRIII,FcRIIIa,FcgammaRIIIA),FcR-10,IgG Fc receptor III-2,FCGR3A
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宿主来源
Mouse抗原名称
CD16分子别名
Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha (Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcgammaRIIIA); FcR-10; IgG Fc receptor III-2; FCGR3A细胞定位
Cell membraneAccession
P08637克隆号
S-R440抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein G浓度
0.2mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5 μl per million cells in 100μl volume | Hu |
CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. These receptors bind to the Fc portion of IgG antibodies, which then activates antibody-dependent cell-mediated cytotoxicity (ADCC) in human NK cells. CD16 is required for ADCC processes carried out by human monocytes. In humans, monocytes expressing CD16 have a variety of ADCC capabilities in the presence of specific antibodies, and can kill primary leukemic cells, cancer cell lines, and cells infected with hepatitis B virus. In addition, CD16 is able to mediate the direct killing of some virally infected and cancer cells without antibodies.
流式分析
- Flow cytometric analysis of Human CD16 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Phycoerythrin Mouse Anti-Human CD56 antibody and either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD16 Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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