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宿主来源
Human抗原名称
CD40分子别名
Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; TNFRSF5细胞定位
Cell membraneAccession
P25942克隆号
ABBV-323抗体类型
Recombinant mAb抗体同种型
IgG1,κ反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.002mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5 μl per million cells in 100μl volume Hu
CD40 is a member of the tumor necrosis factor receptor superfamily and is primarily involved in regulating immune responses. It is highly expressed on cells such as B cells, dendritic cells (DCs), and monocytes, and plays a crucial role in linking innate and adaptive immunity. CD40 signaling is a key trigger in the maturation process of monocytes, mainly driving differentiation into M1-type macrophages and DCs. On DC surfaces, CD40 is involved in promoting the production of cytokines and chemokines, inducing the expression of costimulatory molecules, and enhancing antigen cross-presentation. CD40 is also expressed on various types of cells, including fibroblasts, endothelial cells, and certain hematopoietic and epithelial tumor cells. In the context of CAR-T cell therapy, the expression of CD40L on the surface of CAR-T cells has been explored to enhance their anti-tumor functions.
流式分析
- Flow cytometric analysis of Human CD40 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421 Mouse Anti-Human CD19 antibody and either Alexa Fluor® 647 Human IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Human Anti-Human CD40 Antibody (Right panel) at 0.01 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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