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Rabbit抗原名称
CD19分子别名
B-lymphocyte antigen CD19; LE-CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P15391克隆号
SDT-164-41抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Human tonsil, Human diffuse large B-cell lymphoma纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 555性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
IF ?
稀释度
应用 稀释度 推荐种属 IF 1:200 Hu
The CD19 antigen plays an important role in clinical oncology. In normal cells, it is the most ubiquitously expressed protein in the B lymphocyte lineage. CD19 expression is induced at the point of B lineage commitment during the differentiation of the hematopoietic stem cell, and its expression continues through preB and mature B cell differentiation until it is finally down-regulated during terminal differentiation into plasma cells. CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation, and therefore CD19 is useful in diagnosis of leukemias and lymphomas using monoclonal antibodies (mAbs) and flow cytometry.
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD19 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD19 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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