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Rabbit免疫原
Recombinant Protein抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Species Independent纯化方式
Protein A标记
Agarose beads性状
Liquid缓冲体系
50% slurry in PBS pH7.4, 0.05% Proclin 300. Ensure thorough mixing before each use. Wide-mouth pipette tips are recommended. In case of difficulty in aspiration due to improper operation, additional Storage Buffer can be added at your discretion.储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
Protein purification
Co-IP
IP
Reliable and robust pull-down of mCherry and RFP-fusion proteins. Red fluorescent protein, also known as RFP, is a type of protein that emits red fluorescence under ultraviolet light. It is an essential optical imaging tool in the field of life science. RFP can be used for live cell imaging, marking protein expression, and various protein-related experiments. It is one of the most commonly used fluorescent proteins, along with green fluorescent protein (GFP) and orange fluorescent protein (OFP). RFP has several advantages compared to GFP. Firstly, RFP can be used in combination with GFP to address scientific issues that GFP alone cannot solve. Secondly, RFP has longer excitation and emission wavelengths, which results in a lower background during intracellular imaging.
电泳
Anti-RFP/mcherry agarose beads were used to immunoprecipitated Red fluorescent protein (RFP). During elution with Acidic elution buffer buffer, the enriched RFP fusion protein are released from the agarose beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: RFP fusion protein
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