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产品规格
宿主来源
Mouse抗原名称
CD33分子别名
PWS44, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67细胞定位
MembraneAccession
P20138克隆号
S-R472抗体类型
Mouse mAb抗体同种型
IgG2a,k应用
FCM反应种属 ?
Hu纯化方式
Protein A浓度
0.1mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
稀释度
| 应用 | 稀释度 |
|---|---|
| FCM | 5 μl per million cells in 100μl volume |
背景介绍
CD33 or Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67) is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 can be stimulated by any molecule with sialic acid residues such as glycoproteins or glycolipids. Upon binding, the immunoreceptor tyrosine-based inhibition motif (ITIM) of CD33, present on the cytosolic portion of the protein, is phosphorylated and acts as a docking site for Src homology 2 (SH2) domain-containing proteins like SHP phosphatases. This results in a cascade that inhibits phagocytosis in the cell.
流式分析
- Flow cytometric analysis of Human CD33 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD14 antibody and either Alexa Fluor® 488 Mouse IgG1 Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD33 Antibody (Right panel) at 0.5 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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