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宿主来源
Mouse抗原名称
CD4分子别名
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P01730克隆号
S-574-16抗体类型
Mouse mAb抗体同种型
IgG1应用
FCM反应种属 ?
Hu纯化方式
Protein G浓度
0.2mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用 | 稀释度 |
---|---|
FCM | 5 μl per million cells in 100μl volume |
In molecular biology, CD4 (cluster of differentiation 4) is a glycoprotein that serves as a co-receptor for the T-cell receptor (TCR). CD4 is found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. CD4+ T helper cells are white blood cells that are an essential part of the human immune system. They are often referred to as CD4 cells, T-helper cells or T4 cells. They are called helper cells because one of their main roles is to send signals to other types of immune cells, including CD8 killer cells, which then destroy the infectious particle. If CD4 cells become depleted, for example in untreated HIV infection, or following immune suppression prior to a transplant, the body is left vulnerable to a wide range of infections that it would otherwise have been able to fight.
流式分析
- Flow cytometric analysis of Human CD4 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 antibody and either Alexa Fluor® 700 Mouse IgG1 Isotype Control (Left panel) or SDT Alexa Fluor® 700 Mouse Anti-Human CD4 Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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