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Rabbit抗原名称
Cytokeratin 7分子别名
Cytokeratin-7 (CK-7), Keratin-7 (K7), Sarcolectin, Type-II keratin Kb7, KRT7细胞定位
Cell membrane, IntracellularAccession
P08729克隆号
SDT-R028抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
2.5 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
IF ?
稀释度
应用 稀释度 IF 1:100
Cytokeratin 7 is the intermediate silk protein mainly expressed in epithelial cells. The heterodine of keratin consists of a polymerization filament by an acid -keratin (or type I keratin, keratin 9 to 23) and alkaline keratin (or type II keratin, keratin 1 to 8). Expression similar to but more limited than keratins 8 and 18 in simple, pseudostratified and ductal epithelium, mesothelium and urothelium. It's generally expressed (with some variation) in adenocarcinoma of lung, breast, thyroid, endometrium, cervix, ovary, salivary gland, upper GI tract, urothelial carcinoma, papillary renal cell carcinoma and Paget disease. It's generally negative (with some variation) in colorectal carcinoma, Merkel cell carcinoma, hepatocellular carcinoma, prostatic adenocarcinoma, adrenocortical tumors and squamous cell carcinoma.
免疫荧光
IF shows positive staining in paraffin-embedded human breast cancer. Anti- Cytokeratin 7 (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human gastrointestinal stromal tumor. Anti- Cytokeratin 7 (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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