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宿主来源
Mouse抗原名称
CD45分子别名
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P08575克隆号
S-839-3抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM反应种属 ?
Hu纯化方式
Protein G浓度
0.1mg/ml标记
APC-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| FCM | 2.5 μl per million cells in 100μl volume |
CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
流式分析
Flow cytometric analysis of Human CD45 expression on human PBMC (human peripheral blood mononuclear cell). human PBMC were stained with either APC-Cy7 Mouse IgG1, κ Isotype Control (Black line histogram) or SDT APC-Cy7 Mouse Anti-Human CD45 Antibody (Red line histogram) at 0.25 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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