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宿主来源
Rabbit抗原名称
AMACR/P504S分子别名
Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase免疫原
Synthetic Peptide细胞定位
PeroxisomeAccession
Q9UHK6克隆号
SDT-401-29抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 555性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
IF ?
稀释度
应用 稀释度 IF 1:100-1:200
α-Methylacyl-CoA racemase (AMACR; P504S) catalyzes a key chiral inversion step in the metabolism of branched-chain fatty acids, ibuprofen and related drugs. Protein levels are increased in all prostate and some other cancer cells and it is used as a marker (P504S). The enzyme requires no cofactors and catalyzes its reaction by a stepwise 1,1-proton transfer via an enolate intermediate. The biological role of AMACR in cancer is complex, linking lipid metabolism with nuclear receptor (e.g. FXR and PPAR) activity and expression of enzymes such as cyclooxygenase-2 (COX-2). A number of rationally designed AMACR inhibitors have been reported in the literature as potential cancer treatments. The opportunities and challenges for development of acyl-CoA esters as inhibitors are discussed from a medicinal chemical viewpoint. Other challenges for drug development include the problems in assaying enzymatic activity and the prediction of structure-activity relationships (SAR). [PMID: 23376124]
免疫荧光
IF shows positive staining in paraffin-embedded human colon cancer. Anti- AMACR p504s (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostatic cancer. Anti- AMACR p504s (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse kidney. Anti- AMACR p504s (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse liver. Anti- AMACR p504s (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat kidney. Anti- AMACR p504s (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat liver. Anti- AMACR p504s (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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