Villin Recombinant Rabbit mAb (Alexa Fluor^® 555 Conjugate) (SDT-039-78)

Villin-1,VIL1,VIL

价格￥1,056.00 （供应商现货： 3-5个工作日）

货号 S0B1572

规格

10μl25μl100μl1ml

数量

产品介绍

产品介绍引用文献(1) 评论(0)

产品规格

应用	稀释度
IF	1:200

背景介绍

VIL1 is a gastrointestinal-related cytoskeletal protein that is associated with the microfilament bundles of brush border microvilli. A major structural component of the brush border cytoskeleton, VIL1 binds actin in a calcium-dependent manner. Under normal physiological conditions, villin1 is expressed in epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. Wang et al. report VIL1 to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. Furthermore, the overexpression of villin has been reported in many diseases, such as gastrointestinal neuroendocrine tumors and colon cancer; and in lung cancers, villin1 is reported to be a useful marker to distinguish large cell neuroendocrine carcinomas from squamous cell carcinomas from the sera of patients with lung cancer.

免疫荧光
- IF shows positive staining in paraffin-embedded human colon. Anti-Villin (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human colon cancer. Anti-Villin (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse colon. Anti-Villin (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat colon. Anti-Villin (Alexa Fluor® 555 Conjugate) antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.