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宿主来源
Rabbit抗原名称
TTF-1分子别名
NKX2-1细胞定位
NucleusAccession
P43699克隆号
SDT-R097抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
IF ?
稀释度
应用 稀释度 IF 1:100
背景介绍
Thyroid transcription factor 1 (TTF-1), also known as NKX2-1, is the term preferred by pathologists for this protein; it is also widely used in immunohistochemistry for identifying thyroid or lung as sites of origin for tumours of unknown primary sites.
免疫荧光
IF shows positive staining in paraffin-embedded human thyroid cancer. Anti- TTF-1 (Alexa Fluor® 488 Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.







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