ErbB2 Recombinant Rabbit mAb (Alexa Fluor^® 488 Conjugate) (SDT-069-57)

HER2,MLN19,NEU,NGL,CD340,ERBB2

价格￥1,056.00 （供应商现货： 3-5个工作日）

货号 S0B1567

规格

10μl25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度
IF	1:100

背景介绍

Receptor tyrosine-protein kinase erbB-2 is a protein that in humans is encoded by the ERBB2 gene. ERBB is abbreviated from erythroblastic oncogene B. The human protein is also frequently referred to as HER2 (human epidermal growth factor receptor 2) or CD340 (cluster of differentiation 340). HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. But contrary to other member of the ERBB family, HER2 does not directly bind ligand. HER2 activation results from heterodimerization with another ERBB member or by homodimerization when HER2 concentration are high, for instance in cancer. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years the protein has become an important biomarker and target of therapy for approximately 30% of breast cancer patients.

免疫荧光
- IF shows positive staining in paraffin-embedded human breast cancer (case 1). Anti-ErbB2(Alexa Fluor® 488 Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human breast cancer (case 2). Anti-ErbB2(Alexa Fluor® 488 Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.