所有图片（1/1）
大包装询价 产品介绍 评论(0)
产品规格
宿主来源
Mouse抗原名称
CD235a/b分子别名
Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GYPA, Glycophorin-B, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, GYPB细胞定位
Cell membraneAccession
P02724, P06028克隆号
S-R403抗体类型
Recombinant mAb抗体同种型
IgG2b,k应用
FCM反应种属 ?
Hu纯化方式
Protein A浓度
0.02mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 0.1% BSA, 0.01% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
稀释度
| 应用 | 稀释度 |
|---|---|
| FCM | 5 μl per million cells in 100μl volume |
背景介绍
Glycophorin A (GYPA) and B (GYPB; this protein) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups respectively. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include the Miltenberger (Mi) complex and several isoforms of Stones (Sta); also Dantu, Sat, Henshaw (He or MNS6), Mg and deletion variants Ena, S-s-U- and Mk. Most of these are the result of gene recombinations between GYPA and GYPB.
流式分析
- Flow cytometric analysis of Human CD235a/b expression on K562. Cells from the K562 (Human chronic myelogenous leukemia lymphoblast, Right) or MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Left) was stained with Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Mouse Anti-Human CD235a/b Antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)