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产品规格
宿主来源
Rabbit免疫原
Synthetic Peptide抗体同种型
IgG应用
Protein purification ,IP反应种属 ?
Species Independent标记
Agarose beads缓冲体系
50% slurry in PBS pH7.4, 0.05% Proclin 300. Ensure thorough mixing before each use. Wide-mouth pipette tips are recommended. In case of difficulty in aspiration due to improper operation, additional Storage Buffer can be added at your discretion.储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
背景介绍
Anti-FLAG (DYKDDDDK) Tag agarose Beads is an anti-FLAG Rabbit Recombinant monoclonal antibody covalently conjugated to agarose beads. It can be widely used for immunoprecipitation or purification of fusion proteins or their protein complexes with Flagger tags.
电泳
Anti-FLAG (DYKDDDDK) Tag agarose Beads were used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with Peptide elution buffer, the enriched FLAG fusion protein are released from the agarose beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: FLAG fusion protein.

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