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宿主来源
Mouse抗原名称
CD45分子别名
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P08575克隆号
S-839-3抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
0.1 mg/ml标记
PE性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 FCM 2.5μl per million cells in 100μl volume
CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
流式分析
Flow cytometric analysis of Human CD45 expression on human PBMC (human peripheral blood mononuclear cell). human PBMC were stained with either Phycoerythrin Mouse IgG1, κ Isotype Control (Black line histogram) or SDT PE Mouse Anti-Human CD45 Antibody (Red line histogram) at 0.25 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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