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Mouse抗原名称
CD19分子别名
B-lymphocyte antigen CD19, LE-CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P15391克隆号
S-1039-46抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
RhMk, CyMk, Hu纯化方式
Protein G浓度
0.2mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 FCM 5 μl per million cells in 100μl volume
The CD19 antigen plays an important role in clinical oncology. In normal cells, it is the most ubiquitously expressed protein in the B lymphocyte lineage. CD19 expression is induced at the point of B lineage commitment during the differentiation of the hematopoietic stem cell, and its expression continues through preB and mature B cell differentiation until it is finally down-regulated during terminal differentiation into plasma cells. CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation, and therefore CD19 is useful in diagnosis of leukemias and lymphomas using monoclonal antibodies (mAbs) and flow cytometry.
流式分析
- Flow cytometric analysis of Human CD19 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD3 antibody and either FITC Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD19 Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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