热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 偶联抗体

所有图片（1/10）

Histone H3 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-266-44)

Histone H3.1

价格 ￥1,056.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1521
规格
数量
收藏 分享
产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3

  • 分子别名

    Histone H3.1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-266-44

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Alexa Fluor® 488

  • 性状

    Liquid

  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

  • 应用

    ICFCM

    ICC ?

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    ICC 1:500 Hu, Ms, Rt
    IF 1:500 Hu, Ms, Rt
    ICFCM 1:2000 Hu, Ms, Rt
背景介绍

  • Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3.1 is enriched in PTMs associated with gene activation (K14 acetylation) and gene silencing (K9 dimethylation), suggesting that these mammalian H3 variants may, indeed, have separate biological functions.

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling Histone H3 (Alexa Fluor® 488 Conjugate) antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Histone H3 (Alexa Fluor® 488 Conjugate) antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized C6 (Rat glial tumor glial cell) cells labelling Histone H3 (Alexa Fluor® 488 Conjugate) antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti- Histone H3 (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in NIH/3T3 cells. Anti- Histone H3 (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in C6 cells. Anti- Histone H3 (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human breast cancer. Anti- Histone H3 (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human ovarian cancer. Anti- Histone H3 (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded mouse testis. Anti- Histone H3 (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded rat testis. Anti- Histone H3 (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

引用文献(1)

评论(0)