大包装询价 产品介绍 引用文献(1) 评论(0)
宿主来源
Rabbit分子别名
SMA, Alpha-actin-2, ACTA2免疫原
Synthetic Peptide细胞定位
Cytoskeleton, CytoplasmAccession
P62736克隆号
S-132-63抗体类型
Recombinant mAb抗体同种型
IgG应用
IF反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IF | 1:100 |
α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas Sα-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
免疫荧光
IF shows positive staining in paraffin-embedded human prostate. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse colon. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat colon. Anti-alpha smooth muscle Actin (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
引用文献(1)
- ST-FFPE-mIF: Integrating Spatial Transcriptomics and Multiplex Immunofluorescence in Formalin-Fixed Paraffin-Embedded Tissues Using Stereo-seq
X Zhang, M Zhang, Y Xu, Y Song, X Yang, Y Wu
bioRxiv. 2025 Jan 24 .
货号：S0B1508产品名称：alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (S-132-63)
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