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产品规格
宿主来源
Rat抗原名称
CD3分子别名
T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, Cd3z, Tcrz细胞定位
Cell membraneAccession
P24161克隆号
17A2抗体类型
Recombinant mAb抗体同种型
IgG2b应用
FCM反应种属 ?
Ms纯化方式
Protein G浓度
0.05mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 0.1% BSA, 0.01% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
稀释度
| 应用 | 稀释度 |
|---|---|
| FCM | 5 μl per million cells in 100μl volume |
背景介绍
The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. CD3 is associated with a heterodimer creating the T-cell receptor (TCR). The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines.
流式分析
- Multiparameter flow cytometric analysis of Mouse CD3 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes cells were stained with Alexa Fluor® 647 Rat Anti-Mouse CD19 antibody and either FITC Mouse IgG2b Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse CD3 antibody (Right panel) at 0.1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™software.
实验方案
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