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Rabbit抗原名称
OPN1SW分子别名
Short-wave-sensitive opsin 1; Blue cone photoreceptor pigment; Blue-sensitive opsin (BOP); BCP免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P03999抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human retina, mouse retina, rat retina预测反应种属
(反应种属缩写表)Cz, Mk纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:500 Hu, Ms, Rt IF 1:500 Hu, Rt
OPN1SW, also known as short-wave-sensitive opsin 1 or blue-sensitive opsin, is a protein encoded by the OPN1SW gene in humans. This protein is a type of opsin pigment found in the photoreceptor cells of the retina and is crucial for normal color vision, specifically mediating the perception of blue light. It is a G-protein coupled receptor that initiates the visual transduction cascade by converting light into electrochemical signals. The OPN1SW gene is located on chromosome 7q32.1 and is conserved across several species. Defects in this gene can lead to tritan color blindness, a condition where individuals have impaired blue-yellow color vision.
免疫印迹
WB result of OPN1SW Rabbit pAb
Primary antibody: OPN1SW Rabbit pAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse eye lysate 20 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 40 kDaWB result of OPN1SW Rabbit pAb
Primary antibody: OPN1SW Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat eye lysate 20 µg
Negative control: rat liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 40 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human retina. Anti-OPN1SW antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse retina. Anti-OPN1SW antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human retina. Anti- OPN1SW antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat retina. Anti- OPN1SW antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of OPN1SW in tumor tissue.
Expression of OPN1SW in human tissue.
Expression of OPN1SW in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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