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Rabbit抗原名称
MeCP2分子别名
Methyl-CpG-binding protein 2; MeCp-2 protein免疫原
Synthetic Peptide细胞定位
NucleusAccession
P51608克隆号
S-1884-28抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt阳性样本
SH-SY5Y, Neuro-2a, mouse brain, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt IHC-P 1:500 Hu, Rt
Methyl-CpG-binding protein 2 (MeCP2) is a crucial nuclear protein predominantly found in mature neurons, initially identified as a transcriptional repressor that recognizes DNA methylation marks. It plays a significant role in neurodevelopment, with mutations in the MECP2 gene causing severe neurological disorders such as Rett syndrome (RTT) and MeCP2 duplication syndrome (MDS), which are characterized by progressive neurological dysfunction and developmental regression. MeCP2 exhibits a preference for binding methylated cytosines in both CpG and non-CpG contexts and can also bind unmethylated DNA. It is involved in chromatin organization and can interact with nucleosomes and other effector proteins, such as the NCoR1/2 co-repressor complex, to regulate gene expression. Recent research suggests that MeCP2's functions are more complex than previously thought, with its roles in neurodevelopment and adult brain function linked to its ability to act as a global chromatin regulator.
免疫印迹
WB result of MeCP2 Recombinant Rabbit mAb
Primary antibody: MeCP2 Recombinant Rabbit mAb at 1/500 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 80 kDaWB result of MeCP2 Recombinant Rabbit mAb
Primary antibody: MeCP2 Recombinant Rabbit mAb at 1/500 dilution
Lane 1: Neuro-2a nuclear fraction lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 80 kDaWB result of MeCP2 Recombinant Rabbit mAb
Primary antibody: MeCP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 80 kDaWB result of MeCP2 Recombinant Rabbit mAb
Primary antibody: MeCP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-MeCP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-MeCP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MeCP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-MeCP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MeCP2 in tumor tissue.
Expression of MeCP2 in human tissue.
Expression of MeCP2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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