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Rabbit抗原名称
CBS分子别名
Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P35520抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse kidney, mouse liver, rat kidney, rat liver纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:250 Ms, Rt IF 1:100-1:500 Ms, Rt
Cystathionine beta-synthase (CBS) is a crucial enzyme in the trans-sulfuration pathway that catalyzes the conversion of homocysteine to cystathionine, which is subsequently converted to cysteine. It is a pyridoxal 5′-phosphate (PLP)-dependent enzyme with a modular three-domain structure. The N-terminal domain binds a heme cofactor that acts as a redox sensor, while the C-terminal domain contains a tandem CBS domain that binds S-adenosylmethionine (SAM), an allosteric activator. Mutations in the CBS gene can lead to homocystinuria, a condition associated with cardiovascular, ocular, skeletal, and central nervous system complications. CBS is also involved in hydrogen sulfide (H₂S) production, an important gasotransmitter with various physiological functions.
免疫印迹
WB result of CBS Rabbit pAb
Primary antibody: CBS Rabbit pAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDaWB result of CBS Rabbit pAb
Primary antibody: CBS Rabbit pAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat liver lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat skeletal muscle. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse kidney. Anti-CBS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat kidney. Anti-CBS antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of CBS in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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