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Rabbit抗原名称
IFITM3分子别名
Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b (DSPA2b); Interferon-inducible protein 1-8U免疫原
Synthetic Peptide细胞定位
Endosome, Cell membrane, Cytoplasm, LysosomeAccession
Q01628抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HepG2, THP-1纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
Interferon-induced transmembrane protein 3 (IFITM3) is a small transmembrane protein that plays a crucial role in various cellular functions. It is ubiquitously expressed in most cell types, primarily localized in endosomal and lysosomal membranes, and can also be found in plasma and endoplasmic reticulum membranes. IFITM3 is best known for its antiviral activity, as it can inhibit the entry of a broad spectrum of viruses, including influenza A virus, Zika virus, coronaviruses, and others, by preventing viral membrane fusion with host cells. In addition to its antiviral function, IFITM3 is involved in cell adhesion, stem cell migration, and immune regulation. It has also been implicated in tumor development and progression, as well as in the pathogenesis of diseases like Alzheimer's Disease. The expression of IFITM3 is regulated by interferons, and genetic variations in the IFITM3 gene have been associated with susceptibility to viral infections.
免疫印迹
WB result of IFITM3 Rabbit pAb
Primary antibody: IFITM3 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 15 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-IFITM3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-IFITM3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-IFITM3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IFITM3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of IFITM3 in tumor tissue.
Expression of IFITM3 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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