PDX1 Rabbit Polyclonal Antibody
Pancreas/duodenum homeobox protein 1,PDX-1,Glucose-sensitive factor (GSF),Insulin promoter factor 1 (IPF-1),Insulin upstream factor 1 (IUF-1),Islet/duodenum homeobox-1 (IDX-1),Somatostatin-transactivating factor 1 (STF-1),IPF1,STF1
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宿主来源
Rabbit抗原名称
PDX1分子别名
Pancreas/duodenum homeobox protein 1; PDX-1; Glucose-sensitive factor (GSF); Insulin promoter factor 1 (IPF-1); Insulin upstream factor 1 (IUF-1); Islet/duodenum homeobox-1 (IDX-1); Somatostatin-transactivating factor 1 (STF-1); IPF1; STF1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P52945抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Beta-TC-6预测反应种属
(反应种属缩写表)Gor, Cz纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:1000 Hu, Ms, Rt
PDX1, also known as pancreatic duodenum homeobox-1, is a transcription factor encoded by a Hox-like homeodomain gene that plays a crucial role in pancreatic development, β-cell differentiation, and the maintenance of mature β-cell functions. It was first identified as a nuclear endodermal protein expressed in the epithelium of the duodenum and the pancreas. PDX1 activates the expression of several pancreas-associated genes, including insulin, somatostatin, glucokinase, and others. The PDX1 gene is highly conserved among different species and is composed of two exons encoding a protein of 283 amino acids. It is regulated by numerous distant enhancers and transcription factors, which are essential for pancreatic organogenesis and β-cell function. Research has shown that the overexpression of PDX1 promotes β-cell differentiation and insulin secretion, while its absence increases susceptibility to diabetes. Therefore, PDX1 is considered a promising therapeutic target for treating diabetes.
免疫印迹
WB result of PDX1 Rabbit pAb
Primary antibody: PDX1 Rabbit pAb at 1/1000 dilution
Lane 1: BxPC-3 whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Lane 3: NIH/3T3 whole cell lysate 20 µg
Lane 4: Beta-TC-6 whole cell lysate 20 µg
Negative control: BxPC-3 whole cell lysate; Caco-2 whole cell lysate; NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 40 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human pancreas. Anti-PDX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-PDX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti-PDX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PDX1 in tumor tissue.
Expression of PDX1 in human tissue.
Expression of PDX1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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