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Rabbit抗原名称
PPIF/Cyclophilin F分子别名
Peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; Cyclophilin D (CyP-D; CypD); Mitochondrial cyclophilin (CyP-M); Rotamase F; CYP3免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P30405克隆号
S-2043-61抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HEK-293, HepG2, MCF7, mouse heart, rat heart预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200 Hu ICC 1:500 Hu
PPIF/Cyclophilin F protein is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family, which catalyzes the isomerization of proline imide peptide bonds in oligopeptides, thereby accelerating protein folding. It is a critical component of the mitochondrial permeability transition pore (mPTP) in the inner mitochondrial membrane, and its activation is associated with the induction of apoptotic and necrotic cell death. Cyclophilin F also interacts with mitochondrial p53/TP53 to activate necrosis induced by oxidative stress and regulates mitochondrial membrane F(1)F(0) ATP synthase activity. Additionally, it has anti-apoptotic activity independent of mPTP, cooperating with BCL2 to inhibit cytochrome c-dependent apoptosis.
免疫印迹
WB result of PPIF/Cyclophilin F Recombinant Rabbit mAb
Primary antibody: PPIF/Cyclophilin F Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 23 kDaWB result of PPIF/Cyclophilin F Recombinant Rabbit mAb
Primary antibody: PPIF/Cyclophilin F Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 23 kDaWB result of PPIF/Cyclophilin F Recombinant Rabbit mAb
Primary antibody: PPIF/Cyclophilin F Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 23 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-PPIF/Cyclophilin F antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-PPIF/Cyclophilin F antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-PPIF/Cyclophilin F antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PPIF/Cyclophilin F antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-PPIF/Cyclophilin F antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PPIF/Cyclophilin F in tumor tissue.
Expression of PPIF/Cyclophilin F in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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