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Rabbit抗原名称
SV2A分子别名
Synaptic vesicle glycoprotein 2A; KIAA0736免疫原
Synthetic Peptide细胞定位
SynapseAccession
Q7L0J3克隆号
S-2096-2抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SH-SY5Y, mouse brain, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
Synaptic vesicle glycoprotein 2A (SV2A) is a transmembrane protein found in synaptic vesicles throughout the brain, playing a crucial role in neuronal function. It is involved in regulating neurotransmitter release by modulating the coupling between calcium entry and neurotransmitter release during exocytosis. SV2A also interacts with other synaptic proteins, such as synaptotagmin, and may have additional functions including the transport of galactose and interaction with extracellular matrix components like laminin. Notably, SV2A is the specific target of the anti-epileptic drug levetiracetam, which has led to increased interest in its role in epilepsy and other neurological disorders. Recent studies have also suggested that SV2A may have a mitochondrial localization and function, potentially acting as a fusion or fission factor, which could be relevant to the study of mitochondrial dysfunctions in diseases like Alzheimer’s.
免疫印迹
WB result of SV2A Recombinant Rabbit mAb
Primary antibody: SV2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell unboiled RIPA lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 120-140 kDaWB result of SV2A Recombinant Rabbit mAb
Primary antibody: SV2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain unboiled RIPA lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 110-140 kDaWB result of SV2A Recombinant Rabbit mAb
Primary antibody: SV2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain unboiled RIPA lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 110-140 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human pancreas. Anti-SV2A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SV2A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SV2A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SV2A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SV2A in tumor tissue.
Expression of SV2A in human tissue.
Expression of SV2A in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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