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Phospho-PKR (Thr446) Recombinant Rabbit mAb (S-771-17)

Interferon-induced,double-stranded RNA-activated protein kinase,Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2),Interferon-inducible RNA-dependent protein kinase,P1/eIF-2A protein kinase,Protein kinase RNA-activated (PKR,Protein kinase R),Tyrosine-protein kinase EIF2AK2,p68 kinase,PKR,PRKR,EIF2AK2

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1484
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-PKR (Thr446)

  • 分子别名

    Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2); Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated (PKR; Protein kinase R); Tyrosine-protein kinase EIF2AK2; p68 kinase; PKR; PRKR; EIF2AK2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P19525

  • 克隆号

    S-771-17

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:500 Hu
    ICC 1:100 Hu
背景介绍

  • Protein Kinase R (PKR), also known as double-stranded RNA-dependent protein kinase, is a key enzyme involved in the innate immune response to viral infections. It is an interferon-induced, serine/threonine protein kinase that becomes activated upon binding to double-stranded RNA (dsRNA), which is often produced during viral replication. Once activated, PKR phosphorylates the eukaryotic initiation factor 2α (eIF-2α), inhibiting protein synthesis and thus limiting viral replication. Additionally, PKR plays roles in regulating apoptosis, cell growth, and differentiation, and has been implicated in cancer and metabolic diseases. Its versatile functions make it a significant target for therapeutic research.

  • 免疫印迹

    • WB result of Phospho-PKR (Thr446) Recombinant Rabbit mAb
      Primary antibody: Phospho-PKR (Thr446) Recombinant Rabbit mAb at 1/500 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 62 kDa
      Observed MW: 70 kDa

  • 斑点杂交

    • Dot blot result of Phospho-PKR (Thr446) Recombinant Rabbit mAb
      Lane 1: PKR (Thr446) phospho peptide
      Lane 2: PKR (Thr446) unmodified peptide
      Primary antibody: Phospho-PKR (Thr446) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with Calyculin A (100ng/ml, 30min) and TNF-α (20ng/ml, 5min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-PKR (Thr446) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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