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宿主来源
Rabbit抗原名称
Tau (phospho S214)分子别名
Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); MAPTL; MTBT1; TAU; MAPT免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoskeleton, Endoplasmic reticulum, Secreted, CytoplasmAccession
P10636-8克隆号
S-1032-326抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Ms阳性样本
old mouse brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Ms |
Tau (phospho S214) protein is a phosphorylated form of the tau protein, which is a microtubule-associated protein primarily found in neurons of the central nervous system. It is encoded by the MAPT gene and plays a crucial role in maintaining the stability and promoting the assembly of microtubules in axons. The phosphorylation of tau at serine 214 (S214) by cAMP-dependent protein kinase A (PKA) has been shown to reduce the pathological assembly of the protein. This phosphorylation event is significant as it can influence the protein's binding affinity to microtubules and its involvement in neurofibrillary tangles, which are a hallmark of Alzheimer's disease and other tauopathies.
免疫印迹
WB result of Tau (phospho S214) Recombinant Rabbit mAb
Primary antibody: Tau (phospho S214) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: old mouse brain lysate 20 µg
Lane 2: phosphatase treated old mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 45-55 kDa
斑点杂交
Dot blot result of Tau (phospho S214) Recombinant Rabbit mAb
Lane 1: Tau (S214) phospho peptide
Lane 2: Tau unmodified peptide (around S214)
Primary antibody: Tau (phospho S214) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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