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Rabbit抗原名称
Tau (phospho S214)分子别名
Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); MAPTL; MTBT1; TAU; MAPT免疫原
Synthetic Peptide细胞定位
Cell membrane, Endoplasmic reticulum, Cytoplasm, Cytoskeleton, SecretedAccession
P10636-8克隆号
S-1032-326抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Ms阳性样本
old mouse brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Ms
Tau (phospho S214) protein is a phosphorylated form of the tau protein, which is a microtubule-associated protein primarily found in neurons of the central nervous system. It is encoded by the MAPT gene and plays a crucial role in maintaining the stability and promoting the assembly of microtubules in axons. The phosphorylation of tau at serine 214 (S214) by cAMP-dependent protein kinase A (PKA) has been shown to reduce the pathological assembly of the protein. This phosphorylation event is significant as it can influence the protein's binding affinity to microtubules and its involvement in neurofibrillary tangles, which are a hallmark of Alzheimer's disease and other tauopathies.
免疫印迹
WB result of Tau (phospho S214) Recombinant Rabbit mAb
Primary antibody: Tau (phospho S214) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: old mouse brain lysate 20 µg
Lane 2: phosphatase treated old mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 45-55 kDa
斑点杂交
Dot blot result of Tau (phospho S214) Recombinant Rabbit mAb
Lane 1: Tau (S214) phospho peptide
Lane 2: Tau unmodified peptide (around S214)
Primary antibody: Tau (phospho S214) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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