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Rabbit抗原名称
PYCR1分子别名
Pyrroline-5-carboxylate reductase 1, mitochondrial; P5C reductase 1; P5CR 1免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P32322抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HepG2, PANC-1, A549纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu ICC 1:500 Hu ICFCM 1:500 Hu
PYCR1（pyrroline-5-carboxylate reductase 1）is an enzyme encoded by the PYCR1 gene, located on human chromosome 17q25.3. It catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline, playing a crucial role in proline biosynthesis. PYCR1 is primarily localized in the mitochondria and forms homopolymers. This enzyme may also be involved in the generation of NADP(+) in certain cell types. PYCR1 is broadly expressed in various tissues, including the salivary gland and stomach. It has been implicated in multiple biological processes and diseases. For instance, PYCR1 is overexpressed in many cancers, promoting tumor growth, invasion, and metastasis by supporting proline synthesis and activating various signaling pathways. In addition, it is involved in the regulation of cell proliferation and drug resistance in colorectal cancer through the STAT3-mediated p38 MAPK and NF-κB signaling pathways.
免疫印迹
WB result of PYCR1 Rabbit pAb
Primary antibody: PYCR1 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 31 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) labelling PYCR1 antibody at 1/500 dilution (0.1 μg)/ (red) compared with a Rabbit monoclonal IgG (black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-PYCR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-PYCR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-PYCR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-PYCR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-PYCR1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PYCR1 in tumor tissue.
Expression of PYCR1 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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