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Rabbit抗原名称
Phospho-YAP (Ser127)分子别名
Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP65; YAP1免疫原
Synthetic Peptide细胞定位
Nucleus, Cell membrane, CytoplasmAccession
P46937克隆号
S-1364-28抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu预测反应种属
(反应种属缩写表)Ck, Zf纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:500 Hu, Ms
Phospho-YAP (Ser127) is a phosphorylated form of the Yes-associated protein (YAP), which is a key transcriptional coactivator in the Hippo signaling pathway. YAP regulates various cellular processes, including cell proliferation, survival, differentiation, and organ size. The phosphorylation of YAP at serine 127 by kinases such as MST1/2 and LATS1/2 leads to its inactivation and cytoplasmic retention. When dephosphorylated, YAP can translocate to the nucleus and promote gene transcription. For example, androgen exposure can suppress the inhibitory phospho-Ser127 modification on YAP, resulting in increased YAP nuclear abundance and activity. This regulation is crucial for understanding the role of YAP in diseases like cancer.
免疫印迹
WB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDa
免疫细胞化学
ICC analysis of HeLa cells treated with Calyculin A (100ng/ml,30min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC analysis of NIH/3T3 cells treated with Calyculin A (100nM, 30min) (top panel) and untreated NIH/3T3 cells (below panel). Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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