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MICA/MICB Recombinant Rabbit mAb (S-1589-10)

MHC class I polypeptide-related sequence A,MIC-A,PERB11.1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1477
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    MICA/MICB

  • 分子别名

    MHC class I polypeptide-related sequence A; MIC-A; PERB11.1;

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cell membrane, Endoplasmic reticulum, Cytoplasm

  • Accession

    Q29983, Q29980

  • 克隆号

    S-1589-10

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 阳性样本

    HCT 116, HeLa, A431, MCF7, HUVEC

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu
背景介绍

  • MICA (MHC class I polypeptide-related sequence A) and MICB (MHC class I polypeptide-related sequence B) are stress-inducible proteins that belong to the MHC class I family but do not bind to β2-microglobulin or present peptides. They are encoded by genes located within the HLA class I region of chromosome 6. MICA and MICB are expressed on the cell membrane in response to cellular stress or cancer transformation. These proteins act as ligands for the NKG2D receptor, which is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells. The binding of MICA/B to NKG2D can activate these immune cells, leading to cytotoxic responses against stressed or transformed cells. MICA and MICB are highly polymorphic, with over 106 alleles reported for MICA and 25 for MICB. This polymorphism may affect their affinity for NKG2D and, consequently, the activation thresholds of NK and T cells.

  • 免疫印迹

    • WB result of MICA/MICB Recombinant Rabbit mAb
      Primary antibody: MICA/MICB Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HCT 116 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HeLa treated with PNGase F whole cell lysate 20 µg
      Lane 4: A431 whole cell lysate 20 µg
      Lane 5: MCF7 whole cell lysate 20 µg
      Lane 6: HUVEC whole cell lysate 20 µg
      Lane 7: THP-1 whole cell lysate 20 µg
      Negative control: THP-1 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 43 kDa
      Observed MW: 35-100 kDa

    • WB result of MICA/MICB Recombinant Rabbit mAb
      Primary antibody: MICA/MICB Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Purified human MICB recombinant protein 10 ng
      Lane 2: Purified human MICA recombinant protein 10 ng
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 43 kDa
      Observed MW: 37-70 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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