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DLST Rabbit Polyclonal Antibody

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex,mitochondrial,2-oxoglutarate dehydrogenase complex component E2 (OGDC-E2),Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex,E2K,DLTS

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1475

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

DLST
分子别名

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial; 2-oxoglutarate dehydrogenase complex component E2 (OGDC-E2); Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; E2K; DLTS
免疫原

Synthetic Peptide
细胞定位

Mitochondrion, Nucleus
Accession

P36957
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, HepG2, MCF7, 293T, NIH/3T3, mouse brain, C6, rat brain
预测反应种属
(反应种属缩写表)

Bv, Pg
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200-1:500 Hu
ICC 1:500 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200-1:500	Hu
ICC	1:500	Hu
ICFCM	1:50	Hu

背景介绍

DLST (dihydrolipoamide S-succinyltransferase) is a protein-coding gene located on chromosome 14 that encodes a mitochondrial protein belonging to the 2-oxoacid dehydrogenase family. This protein is the E2 component of the 2-oxoglutarate dehydrogenase complex, which catalyzes the conversion of 2-oxoglutarate to succinyl-CoA and CO₂, playing a crucial role in the citric acid cycle. DLST is expressed in various tissues, including the kidney and heart. Mutations in the DLST gene have been associated with diseases such as pheochromocytoma/paraganglioma syndrome.

免疫印迹
- WB result of DLST Rabbit pAb
  Primary antibody: DLST Rabbit pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Lane 4: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 49 kDa
  Observed MW: 50 kDa
- WB result of DLST Rabbit pAb
  Primary antibody: DLST Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 49 kDa
  Observed MW: 50 kDa
- WB result of DLST Rabbit pAb
  Primary antibody: DLST Rabbit pAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 49 kDa
  Observed MW: 50 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling DLST antibody at 1/50 dilution (1 μg)/ (red) compared with a Rabbit monoclonal IgG (black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-DLST antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-DLST antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-DLST antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-DLST antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-DLST antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-DLST antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of DLST in tumor tissue.
- Expression of DLST in human tissue.
- Expression of DLST in mouse & rat tissue.