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DLAT Rabbit Polyclonal Antibody

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex,mitochondrial,70 kDa mitochondrial autoantigen of primary biliary cirrhosis (PBC),Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex,M2 antigen complex 70 kDa subunit,Pyruvate dehydrogenase complex component E2 (PDC-E2,PDCE2),DLTA

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1474

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

DLAT
分子别名

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis (PBC); Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; Pyruvate dehydrogenase complex component E2 (PDC-E2; PDCE2); DLTA
免疫原

Synthetic Peptide
细胞定位

Mitochondrion
Accession

P10515
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, HepG2, LNCaP, A431, NIH/3T3, mouse brain, C6, rat brain
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200-1:500 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200-1:500	Hu, Ms, Rt

背景介绍

Dihydrolipoamide S-acetyltransferase (DLAT) is a key component of the pyruvate dehydrogenase complex (PDC), which is located in the mitochondria. This enzyme plays a crucial role in cellular energy production by facilitating the conversion of pyruvate into acetyl-CoA, a process that links glycolysis to the citric acid cycle. DLAT accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A (CoA). It is encoded by the DLAT gene, which is conserved across various species. Mutations in this gene can lead to pyruvate dehydrogenase E2 deficiency, causing primary lactic acidosis in infancy and early childhood. Additionally, DLAT is the antigen for antimitochondrial antibodies, which are present in nearly 95% of patients with primary biliary cirrhosis (PBC), an autoimmune liver disease.

免疫印迹
- WB result of DLAT Rabbit pAb
  Primary antibody: DLAT Rabbit pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: LNCaP whole cell lysate 20 µg
  Lane 4: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 69 kDa
- WB result of DLAT Rabbit pAb
  Primary antibody: DLAT Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 69 kDa
- WB result of DLAT Rabbit pAb
  Primary antibody: DLAT Rabbit pAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 69 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human stomach. Anti-DLAT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-DLAT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-DLAT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-DLAT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-DLAT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-DLAT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of DLAT in human tissue.
- Expression of DLAT in tumor tissue.
- Expression of DLAT in mouse & rat tissue.